Organogénesis indirecta en fresa (Fragaria x ananassa Duch.) usando dos tipos de explantes
DOI:
https://doi.org/10.57188/manglar.2026.022Keywords:
Callo, micropropagación, regeneración, reguladores de crecimientoAbstract
La propagación in vitro es importante por generar producción masiva de plantas homogéneas, libres de enfermedades y de gran calidad. El objetivo fue desarrollar un protocolo de organogénesis indirecta en fresa. Se usó dos explantes: hojas cotiledonares e hipocótilo. En desinfección se usaron tres concentraciones de hipoclorito de sodio (0,9; 1,1 y 1,3 %); en inducción a callos, se utilizó el medio basal MS con 2,4 D (0,2 mg. L-1 y 1 mg. L-1) y BAP (0,1 mg. L-1) o TDZ (1 mg. L-1); y, en regeneración de brotes, el medio basal MS con BAP (2 mg. L-1), TDZ (0,5 mg. L-1) y 2,4 D (0,2 mg. L-1) o ANA (0,2 mg. L-1). El diseño estadístico fue factorial 2x3 Completamente al azar. Los resultados, en hojas cotiledonares e hipocótilo, indicaron que el NaClO al 1,3% fue el más apropiado en desinfección, mientras que, el medio para inducir mayor porcentaje de callos fue con 0,2 mg. L-1 2,4 D y 1 mg. L-1 TDZ. Los mayores porcentajes de regeneración de brotes en hojas cotiledonares e hipocótilo fueron con los tratamientos de 2,4D y ANA respectivamente. El protocolo obtenido se constituye en un instrumento de valor para futuras investigaciones.
Downloads
References
Aboksari, H. A., Zeinalabedini, M., Nalousi, A. M., Hashemidehkordi, E., & Azadi, P. (2026). Micropropagation and flowering of calla Lily via proliferative basal cluster enhancement by fipexide. Plant Cell, Tissue and Organ Culture, 164, 69. https://doi.org/10.1007/s11240-026-03372-8
Agudelo-Morales, C., Lerma T., Martínez Lara J., Palencia, M., & Combatt, E. (2021). Phytohormones and Plant Growth Regulators—A Review. Journal of Science with Technological Applications, 10, 27–65. https://doi.org/10.34294/j.jsta.21.10.66
Akter, F., Wu, S., Islam, S., Kyaw, H., Yang, J., Li, M., Fu, Y., & Wu, J. (2024). An efficient Agrobacterium-Mediated Genetic Transformation System for Gene Editing in Strawberry (Fragaria x ananassa). Plants, 13(5), 563. https://doi.org/10.3390/plants13050563
Alcantara, J. S., Acero, J., Alcántara, J., & Sánchez, R. (2019). Principales reguladores hormonales y sus interacciones en el crecimiento vegetal. Nova, 17(32), 109–129.
Arias, F., Appelmann, T., Fernando, L., & Garcés, L. (2020). Import and export of strawberries in a global market. In: Sepúlveda, J.A. (ed.). Ciencias económicas y contables, desafíos y retos para la competitividad. Coruniamericana. Colombia. pp. 280-291.
Banerjee, D., Singh, V., & Thakar, R. (2023). Micro propagation on strawberry: A Review. E3S Web of Conferences, 453, 01019. https://doi.org/10.1051/e3sconf/202345301019
Bidabadi, S. S., & Jain, S. M. (2020). Cellular, Molecular, and Physiological Aspects of in Vitro Plant Regeneration. Plants, 9(69), 702. https://doi.org/10.3390/plants9060702
Cappelletti, R., Sabbadini, S., & Mezzetti, B. (2016). The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Scientia Horticulturae, 207, 117–124. https://doi.org/10.1016/j.scienta.2016.05.016
Chomboon, P., & Lohasupthawee, P. (2022). Mass propagation system of strawberry (Fragaria x ananassa) micro shoots by liquid shake culture. Current Applied Science and Technology, 22(1). https://doi.org/10.55003/cast.2022.01.22.008
Chung, H. H., & Ouyang, H. Y. (2021). Use of Thidiazuron for High-Frequency Callus Induction and Organogenesis of Wild Strawberry (Fragaria vesca). Plants, 10(1), 67. http://dx.doi.org/10.3390/plants10010067
Córdova, A. M., Cobos, M., Imán, S. A., & Castro, J. C. (2014). Un método eficiente para la inducción de callos in vitro en Myrciaria dubia (Kunth) Mc Vaugh “Camu Camu”. Scientia Agropecuaria, 5(1), 25–34. https://doi.org/10.17268/sci.agropecu.2014.01.03
Du, Y., Zhao, W., Xin, M., Li, S., Zhu, L., Ma, Q. et al. (2026). In vitro regeneration of Acer truncatum via shoot organogénesis from stem explants. Plant Cell, Tissue and Organ Culture, 164, 4. https://doi.org/10.1007/s11240-025-03321-x
Espinosa, A., Silva, J., Sariego, S., Cholo, L., & Delgado, H. (2012). Efecto del tipo de explante y la concentración de ácido 2,4-diclorofenoxiacético en la formación de callos en Morus alba L. Pastos y Forrajes, 35(4), 407–416.
Food and Agriculture Organization - FAO. 2021. FAOSTAT. Disponible en: http://www.fao.org/faostat/en/#data/QC [Accesado el 8 de abril de 2023]
García-Carrasco, G. A. (2022). Organogénesis indirecta a partir de segmentos de hojas cotiledonares e hipocótilo de Fresa (Fragaria x ananassa Duchesne 1766), Piura. Tesis de Grado. Escuela Profesional de Ciencias Biológicas. Facultad de Ciencias. Universidad Nacional de Piura, Piura, Perú.
George, E. F., Hall, M. A., & Klerk, G. J. (2007). Plant Propagation by Tissue Culture. Springer. Netherlands. https://doi.org/10.1007/978-1-4020-5005-3
Gupta, S. (2022). In Vitro Propagation and Conservation of Fragaria Species. En: Recent Studies on Strawberries. IntechOpen. https://doi.org/10.5772/intechopen.103095
Hasan, N., Nigar, S., Rabbi, M. A. K., Mizan, S. B., & Rahman, M. S. (2010). Micropopagation of Strawberry (Fragaria x ananassa Dutch.). Journal of Crop Production, 5, 36–41.
Hernández Ruiz, J., Rangel Castillo, A. E., Laguna Estrada, M. I., Alejandro Rojas, G. I., Mireles Arriaga, A. I., & Ruiz Nieto. J. E. (2025). Plantas de fresa regeneradas in vitro mediante organogénesis directa en diferentes concentraciones de auxinas y citocininas. Bioagro, 37(1), 123-134. https://doi.org/10.51372/bioaagro371.11
Hernández-Villalobos, K., & Chico-Ruíz, J. (2020). Inducción de brotes y raíces en hipocotilos y cotiledones de Physalis peruviana L. utilizando 6-bencilaminopurina y 2,4-diclorofenoxiacético. Revista de Investigaciones Altoandinas-Journal of High Andean Research, 22(1), 87–94. https://doi.org/10.18271/ria.2020.539
Jhajhra, S., Dashora, L. K., Singh, J., Bhatnagar, P., Kumar, A., & Arya, C. K. (2018). In vitro propagation of strawberry (Fragaria x ananassa Duch.). International Journal of Current Microbiology and Applied Sciences 7(10), 3030-3035. https://doi.org/10.20546/ijcmas.2018.710.353
Joshi, S., Nath, J., Bagri, J., Kumari, K., Roy, A., Yaday, S. K. et al. (2026). High- efficiency regeneration and transformation system in Nardostachys jatamansi for functional genomics and bioactive compounds enrichment. Plant, Cell & Environment, 49(2), 840-857. https://doi.org/10.1111/pce.70263
Karmaker, S., Hossain, Md. M., Hoque, Md. A., Kaium, Md. A., Alí, T. B., & Ferdous, M. (2023). In vitro propagation of three strawberry varieties and field evaluation. American Journal of Plant Sciences, 14, 1214-1222. https://doi.org/10.4236/ajps.2023.1411082
Kim, J., Yoo, C., Nguyen, C. D., Lee, M. B., Huo, H., & Lee, S. (2024). Optimization of shoot regeneration and Agrobacterium-mediated transformation in strawberry (Fragaria x ananassa). Journal of the American Society for Horticultural Science, 150(1), 10-18. https://doi.org/10.21273/JASHS05388-24
Landi, L., & Mezzetti, B. (2006). TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. Plant Cell Reports, 25(4), 281–288. https://doi.org/10.1007/s00299-005-0066-5
Li, X., Liu, L., Luo, C., Chen, Z., & Shu, B. (2026). Efficient CRISPR/Cas9 system established via co-cultivation of plantlets and Agrobacterium tumefaciens for positive transgenic calluses generation and regeneration in cultivated strawberry (Fragaria x ananassa). Plant Physiology and Biochemistry, 232, 111195. https://doi.org/10.1016/j.plaphy.2026.111195
Li, Y., Huang, P., Qiu, X., Zhu, F., Chen, H., Wang, S. et al. (2025). Integration of green and far-red light with red-blue light enhances shoot multiplication in micropropagated strawberry. Horticulturae, 11, 701. https://doi.org/10.3390/horticulturae11060701
Lin, D., Yang, Y., Khalil, R., Xian, Z., Hu, G., & Li, Z. (2013). SlmiR393 controls the auxin receptor homologous genes expression and regulates sensitivity to auxin in tomato root growth. Scientia Horticulturae, 162, 90–99. https://doi.org/10.1016/j.scienta.2013.07.028
Long, Y., Yang, Y., Pan, G., & Shen, Y. (2022). New Insights into Tissue Culture Plant-Regeneration Mechanisms. Frontiers in Plant Science, 13, 926752. https://doi.org/10.3389/fpls.2022.926752
Lu, F., Li, Z., Liu, Y., Zhang, D., Liu, L., Li, D., et al. (2026). Establishment in vitro regeneration and Agrobacterium-mediated genetic transformation system for Lilium longiflorum ‘Sally’ stem segments. Scientia Horticulturae, 363, 114882. https://doi.org/10.1016/j.scienta.2026.114882
Mamani-Sánchez, B., & Murillo-García, R. A. (2020). Micropropagación de dos variedades de frutilla (Fragaria ananassa Duch.) en diferentes medios de cultivo. Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales, 7(1), 69–78.
Martín, R., Chong-Pérez, B., & Pérez-Alonso, N. (2015). Organogénesis in vitro en el género Digitalis. Biotecnología Vegetal, 15(4), 105-206.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497. http://dx.doi.org/10.1111/j1399-3054.1962.tb08052.x
Naing, A. H., Kim, S. H., Chung, M. Y., Park, S. K., & Kim, C. K. (2019). In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars. Plants Methods, 15, 36. https://doi.org/10.1186/s13007-019-0421-0
Nalini, P., Rai, R., Srivastava, R., Arora, S., & Guru, S. K. (2021). An efficient protocol for in vitro propagation of strawberry cv. Chandler through shoot tip culture. Progressive Horticulturre, 53(1). https://doi.org/10.5958/2249-5258.2021.00004.X
Neri, J. C., Meléndez-Mori, J. B., Tejada-Alvarado, J. J., Vilca-Valqui, N. C., Huamán-Huamán, E., Oliva, M., & Goñas, M. (2022). An optimized protocol for micropropagation and acclimatization of strawberry (Fragaria x ananassa Duch.) variety ‘Aroma’. Agronomy, 12(4), 968. https://doi.org/10.3390/agronomy12040968
Omidi, M., & Yadollahi, A. (2016). Factors affecting in vitro propagation efficiency of three genotype of Rosa damascena. Journal of Biology and Nature, 6, 78–87.
Palei, S., Rout, G. R., Das, A. K., & Dash, D. K. (2017). Callus Induction and Indirect Regeneration of Strawberry (Fragaria × Ananassa) Duch. cv Chandler. International Journal of Current Microbiology and Applied Sciences, 6(11), 1311–1318. https://doi.org/10.20546/ijcmas.2017.611.157
Palei, S., Digbijaya, S., Suchismita, J., Ankita, M., Mahipal, S. K., Snehasish, R. et al. (2025). Optimization of in vitro regeneration protocol for Fragaria x ananassa Duch cv Chandler and assessment of genetic fidelity through ISSR markers. Plant Science Today, 12(3). https://doi.org/10.14719/pst.6585
Ramirez, L. A., Moreno, J. E., & González, N. E. (2014). Evaluación del efecto de tratamientos de desinfección con hipoclorito de sodio sobre segmentos nodales de Guadua angustifolia Kunth para el establecimiento del cultivo in vitro. Revista de Investigación Agraria y Ambiental, 5(1), 155-169. https://doi.org/10.22490/21456453.944
Ramlal, A., Quan, P. W., Rajendran, A., & Subramaniam, S. (2025). Impact of biophysicochemical factors on micropropagation, haploidy and doubled haploidy in strawberry (Fragaria sp.): A critical revisit. Current Plant Biology, 44, 100555. https://doi.org/10.1016/j.cpb.2025.100555
R Development Core Team. (2020). R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/
Redhwan, A., Acemi, A., & Özen, F. (2026). In vitro propagation of Fritillaria pontica Wahlenb. Plant Cell, Tissue and Organ Culture, 164, 100. https://doi.org/10.1007/s11240-026-03408-z
Sahu, P. K., Bandyopadhyay, S., & Gantait, S. (2026). Indirect organogenesis and genetic fidelity assessment of small potato (cultivar Badami alu). Journal of Crop Science and Biotechnology, 29, 343-352. https://doi.org/10.1007/s12892-025-00340-0
Sharma, T., Sharma, S., Chib, S., Kaul, S., & Dhar, M. K. (2026). Establishment of a platform for Agrobacterium mediated genetic transformation in Plantago ovate Forssk. Plant Cell, Tissue and Organ Culture, 164, 99. https://doi.org/10.1007/s11240-026-03405-2
Silva, J. A. T, Winarto, B., Dobránszki, J., & Zeng, S. (2015). Disinfection procedures for in vitro propagation of Anthurium. Folia Horticulturae, 27(1), 3–14. https://doi.org/10.1515/fhort-2015-0009
Ugur, R. (2020). Development of in vitro sterilization protocol for do-1 (Prunus domestica) rootstock. Applied Ecology and Environmental Research, 18(2), 2339-2349. https://doi.org/10.15666/aeer/1802_23392349
Wang X., Tang, L., Fan, Y., Luo, X., & Zeng, D. (2017). Organogenesis Approaches in Tissue Culture of Stem Tips of Benihoppe Strawberry (Fragaria ananassa Duch.). Journal of Sichuan Forestry Science and Technology, 34(6), 40–44. https://doi.org/10.16779/j.cnki.1003-5508.2017.06.010
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Gabriela Alexandra García-Carrasco, Arturo Adolfo Arbulú-Zuazo, Henry Robles-Cueva, Humberto Rivera-Calle, Luis Conrado Guzmán-Farfán
This work is licensed under a Creative Commons Attribution 4.0 International License.
Manglar is an open access journal distributed under the terms and conditions of Creative Commons Attribution 4.0 International license
