Yield and content of cannabidiol (CBD) and tetrahydrocannabinol (THC) in medicinal cannabis (Cannabis sativa) grown in the Ecuadorian highlands
DOI:
https://doi.org/10.57188/manglar.2024.011Abstract
Cannabis is an herbaceous species of the Cannabaceae family and is native to the Himalayas. It contains hundreds of metabolites with potential bioactivity, including cannabinoids, terpenes, and flavonoids. The objective of this experiment was to evaluate the yield and the cannabidiol (CBD) and tetrahydrocannabinol (THC) content of medicinal cannabis (variety Cherry Oregon) in two localities of the Ecuadorian highlands. A Randomized Complete Block Design with four treatments and four replications was carried out. Treatment 1 was maintained with natural light, treatment 2 received 16/8 photoperiod (16 hours light, 8 hours dark) for one week, treatment 3 received 16/8 photoperiod for two weeks, and treatment 4 received 16/8 photoperiod for 3 weeks. In the locality 1, treatment 4 showed better values of plant height, dry biomass, biomass yield per plant and greater biomass yield per m2, in both cycles. In the locality 2, the treatments were statistically similar in all variables in both cycles, but a trend where treatment 4 presented higher values compared to the rest of the treatments was observed. The THC and CBD contents for the treatments and cycles ranged between 0.06% and 0.51%, and between 13.76% and 15.29% respectively in both localities. Finally, the results obtained agree with what is reported in the variety's technical sheet, and the THC content does not exceed the maximum value allowed by Ecuadorian regulations.
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Andre, C. M., Hausman, J. F., & Guerriero, G. (2016). Cannabis sativa: the plant of the thousand and one molecules. Frontiers in Plant Science, 7, 19. https://doi.org/10.3389/fpls.2016.00019
Ángeles López, G., Brindis, F., Cristians Niizawa, S., & Ventura Martínez, R. (2014). Cannabis sativa L., una planta singular. Revista mexicana de ciencias farmacéuticas, 45(4), 1-6.
Arguedas-Gamboa, M., Rodríguez-Solís, M., Guevara-Bonilla, M., Esquivel-Segura, E., Sandoval-Rocha, S., & Briceño-Elizondo, E. (2019). Incidencia y severidad de Olivea tectonae y Rhabdopterus sp. en plantaciones jóvenes de Tectona grandis bajo distintas modalidades de control de arvenses. Agronomía Costarricense. 43(1), 9-19.
Arsenault, T. L., Prapayotin-Riveros, K., Ammirata, M. A., White, J. C., & Dimkpa, C. O. (2024). Compliance Testing of Hemp (Cannabis sativa L.) Cultivars for Total Delta-9 THC and Total CBD Using Gas Chromatography with Flame Ionization Detection. Plants, 13(4), 519.
Azcón-Bieto, J., & Talón, M. (2000). Fundamentos de Fisiología Vegetal. McGrawHill Interamericana, 2-4.
Bart, B. (2017). Cultivo para novatos. Soft Secrets, 12-15.
Bernstein, N., Gorelick, J., & Koch, S. (2019). Interplay between chemistry and morphology in medical cannabis (Cannabis sativa L.). Industrial Crops and Products. 129, 185–194. https://doi.org/10.1016/j.indcrop.2018.11.039
Brenneisen, R. (2007). Chemistry and analysis of phytocannabinoids and other cannabis constituent. En: ElSohly, Mahmoud A editores. Marijuana and the cannabinoids. EE. UU.: Humana Press Inc.
Cermeño, Z. S. (2005). Construcción de invernaderos. Mundi-Prensa Libros, 29-50.
Código Orgánico Integral Penal, Suplemento – Registro Oficial No. 107, martes 24 de diciembre del 2019. Ecuador, Quito.
Chandra, S., Lata, H., Khan, I. A., & ElSohly, M. A. (2017). Cannabis sativa L.: botany and horticulture. in Cannabis sativa L.–Botany and biotechnology. ed. S. Chandra (New York, NY: Springer), 79–100.
Danziger, N., & Bernstein, N. (2022). Too Dense or Not Too Dense: Higher Planting Density Reduces Cannabinoid Uniformity but Increases Yield/Area in Drug-Type Medical Cannabis. Front Plant Sci., 13, 713481. https://doi.org/10.3389/fpls.2022.713481
De la Fuente JR (2015). Botánica, química y farmacología de cannabis sativa. En: Marihuana y salud. Facultad de Ciencias Económicas, Universidad Nacional Autónoma de México, Facultad de Medicina/Academia Nacional de Medicina.
Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M., & Robledo, C. W. (2017). Grupo InfoStat, Facultad de Ciencias Agrarias, Universidad Nacional de Córdoba, Córdoba, Argentina.
Fundación Daya. (2020). Cannabis medicinal: una breve guía sobre usos y efectos. Consultado el: 24 de septiembre de 2020. http://www.fundaciondaya.org/cannbis-medicnal-usos-efectos-tipos-de-cannabis/
Gezovitch, O., Luria, N., Lachman, O., Sela, N., Smith, E., & Dombrovsky, A. (2024). Cucurbit chlorotic yellows virus, a crinivirus infecting Cannabis sativa plants. Plant Pathology, 73(1), 47-56.
Gorelick, J., & Bernstein, N. (2014). Elicitation: an underutilized tool in the development of medicinal plants as a source of therapeutic secondary metabolites. In Advances in agronomy (Amsterdam: Elsevier Inc). 201–230. https://doi.org/10.1016/B978-0-12-800138-7.00005-X
Grof, C. P. L. (2018). Cannabis, from plant to pill. J. Clin. Pharmacol. 84, 2463–2467. https://doi.org/10.1111/bcp.13618
Islam, M.M.; Rengel, Z.; Storer, P.; Siddique, K.H.M.; Solaiman, Z.M. (2022) Industrial Hemp (Cannabis sativa L.) Varieties and Seed Pre-Treatments Affect Seed Germination and Early Growth of Seedlings. Agronomy. 12(1), 6. https://doi.org/10.3390/agronomy12010006
Janatová, A., Fraňková, A., Tlustoš, P., Hamouz, K., Božik, M., & Klouček, P. (2018). Yield and cannabinoids contents in different cannabis (Cannabis sativa L.) genotypes for medical use. Industrial Crops and Products, 212, 363-367. https://doi.org/10.1016/j.indcrop.2017.12.006.
Lachenmeier, D.W., Kroener, L., Musshoff, F., & Madea, B. (2004). Determination of cannabinoids in hemp products by headspace solid-phase micro-extraction and gas chromatography mass spectrometry. Anal. Bioanal. Chem 378, 183 – 189.
Lavilla, M., & Ivancovich, A. (2016). Propuestas de escalas para la evaluación, a campo y en laboratorio, del “tizón foliar” y la “mancha púrpura de la semilla”, causadas por Cercopora kikuchii, en soja. INTA. Buenos Aires: argentina. 7 pp.
Merino, J., Pedreros, A., Fischer, S., & López, M. D. (2019). Critical period of weed interference on total polyphenol content in quinoa. Chilean journal of agricultural research, 79(3), 405-414. https://doi.org/10.4067/S0718-58392019000300405
Koo, Y. M., Ahsan, S. M., Kwon, D. B., et al. (2023). Quantitative analysis of cannabidiol and Δ9-tetrahydrocannabinol contents in different tissues of four cannabis cultivars using gas chromatography-mass spectrometry. Korean Journal of Horticultural Science and Technology, 41(3), 339-348. http://dx.doi.org/10.7235/HORT.20230031
Monton, C., Tanpao, T., Navakul, C., et al. (2023). Cannabidiol, ∆ 9-tetrahydrocannabinol, and cannabinol contents of Cannabis sativa L. inflorescences claimed to be Hang Kra Rog Phu Phan cultivar cultivated outdoors in various locations of Thailand. Phytochemistry Letters, 57, 126-132. https://doi.org/10.1016/j.phytol.2023.08.009
Morgan, W., Singh, J., Kesheimer, K., Davis, J., & Sanz-Saez, A. (2024). Severe drought significantly reduces floral hemp (Cannabis sativa L.) yield and cannabinoid content but moderate drought does not. Environmental and Experimental Botany, 219, 105649. https://doi.org/10.1016/j.envexpbot.2024.105649
Nascimento, N. C., & Fett-Neto, A. G. (2010). Plant secondary metabolism and challenges in modifying its operation: An overview. In Plant secondary metabolism engineering. Methods in molecular biology. Ed. Fett-Neto, A. G. 1–13.
Punja, Z. (2018). Flower and foliage-infecting pathogens of marijuana (Cannabis sativa L.) plants. Canadian Journal of Plant Pathology, 40(4), 514-527
Rifna E.J., Ratish Ramanan, K., & Mahendran, R. (2019). Emerging technology applications for improving seed germination. Trends in Food Science & Technology, 86, 95-108, https://doi.org/10.1016/j.tifs.2019.02.029.
Saloner, A., & Bernstein N. (2022). Effect of Potassium (K) Supply on Cannabinoids, Terpenoids and Plant Function in Medical Cannabis. Agronomy, 12(5), 1242. https://doi.org/10.3390/agronomy12051242
Schoepke, T. (2014). Cultivo de marihuana: Manual básico de agricultura. 4-30.
Small, E. (2015). Evolution and classification of Cannabis sativa (Marijuana, Hemp) in relation to human utilization. Bot. Rev. 81, 189–294. https://doi.org/10.1007/s12229-015-9157-3.
Tamosiunas, G., Pagano, E., & Artagaveytia, P. (2013). Una introducción al perfil farmacológico y terapéutico de la marihuana. Arch Med Int, 35, 113-116.
Troya, C., & Vaca Granda, L. (2016). Manual para la Red Nacional de Vigilancia Fitosanitaria. Agencia Ecuatoriana de Aseguramiento de Calidad del Agro. Quito: Ecuador. 97 pp.
Unicef. (2020). La salud mental es determinante para que niños, niñas, adolecentes, familias y comunidades puedan salir adelante. https://www.unicef.org/ecuador/comunicados-prensa/la-salud-mental-es-determinante-para-que-niños-niñas-adolescentes-familias-y
Villagrán, E. (2016). Diseño y evaluación climática de un invernadero para condiciones de clima intertropical de montaña. Tesis de Maestría en Ingeniería - Ingeniería de Biosistemas. Universidad Nacional de Colombia.
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