Efectos de la co-inoculación de Bioformulados (PGPR´s) sobre el porcentaje de germinación y promover el crecimiento en plántula de papaya (Carica papaya L.)
DOI:
https://doi.org/10.57188/manglar.2023.017Resumen
La latencia es un bloqueo para la germinación y representa un mecanismo adaptativo que permite a la semilla de papaya retrasar su emergencia. Las rizobacterias mejoran el crecimiento de las plantas de diversas formas, la producción de fitohormonas, la fijación de nitrógeno, la solubilización de fosfatos y aumenta la morfología de las raíces. La presente investigación se realizó para determinar la eficiencia de las rizobacterias (PGPR´s) Pseudomona protegens (CHA0) y Pseudomona putida (BMR 2-4) inoculadas dentro de tres bioformulados para ser evaluadas dentro del porcentaje de germinación, incremento de pelos absorbentes utilizando rizotrones y plántulas bajo consorcios o en combinación de fertilizantes fosforados. En condiciones in-vitro la co-inoculación BIOIMPULSE fue muy eficaz para aumentar el porcentaje de germinación con promedio 80%. En rizotrones se exhibieron mayor aumento de pelos absorbentes de 5,67 mm y área de raicillas 0,86 mm2 por BIOIMPULSE comparable al tratamiento expuesto con AIA (25 mg/mL). En plántulas son altamente efectivas para mejorar la morfología de las raíces y altura emendando 5 g/L fosforo se observó dimensiones Ø del hipocótilo entre (5,12 a 4,62 mm) y hojas funcionales (9,75 a 11,75). BIOIMPULSE modifican el funcionamiento de las raíces, mejoran la nutrición de las plantas e influyen en la fisiología vegetativa siendo versátil sobre todos los demás bioformulados por aumentar el porcentaje de germinación, aparición temprana de pelos radicular e incremento radicular en presencia de fertilizantes fosforados. Lo que repercute que pueden ser útiles para reducir el costo de los fertilizantes químicos.
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Adedayo, A. A., Babalola, O. O., Prigent-Combaret, C., Cruz, C., Stefan, M., Kutu, F., & Glick, B. R. (2022). The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: A review. PeerJ, 10, e13405.
Adoko, M. Y. E., Agbodjato, N. A., Noumavo, A. P., Amogou, O. E., Adjanohoun, A., & Baba-Moussa, L. (2021). Bioformulations based on plant growth promoting rhizobacteria for sustainable agriculture: Biofertilizer or Biostimulant?. African Journal of Agricultural Research, 17(9), 1256-1260. https://doi.org/10.5897/AJAR2021.15756.
Alara, O. R., Abdurahman, N. H., & Alara, J. A. (2020). Carica papaya: comprehensive overview of the nutritional values, phytochemicals and pharmacological activities. Advances in Traditional Medicine, 22(1), 1-31.
Benítez, S. P., Mario, L., Delgado, O. A., & Medina, C. I. (2013). Estudios de germinación y remoción de latencia en semillas de papayuelas Vasconcellea cundinamarcensis y Vasconcellea goudotiana. Ciencia y Tecnología Agropecuaria, 14(2), 187-197. https://doi.org/10.21930/rcta.vol14_num2_art:407.
Bharathi, R., Vivekananthan, R., Harish, S., Ramanathan, A., & Samiyappan, R. (2004). Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Protection, 23(9), 835-843. https://doi.org/10.1016/j.cropro.2004.01.007.
Cardarelli, M., Woo, S. L., Rouphael, Y., & Colla, G. (2022). Seed Treatments with Microorganisms Can Have a Biostimulant Effect by Influencing Germination and Seedling Growth of Crops. Plants, 11(3), 259. https://doi.org/10.3390/plants11030259.
Carrasco-Fernández, J., Guerra, M., Castro, J. F., Bustamante, L., Barra-Bucarei, L., Ceballos, R., ... & France, A. (2020). Plant growth promoting rhizobacteria from Juan Fernández archipelago improve germination rate of endangered plant Solanum fernandezianum Phil. Chilean journal of agricultural research, 80(1), 41-49. http://dx.doi.org/10.4067/S0718-58392020000100041.
Chandni, B., Kanzaria, D. R., Karmur, N. N., Sandh, M., Parsana, J. S., & Patel, H. N. (2022). Effect of sarcotesta on papaya seedling. The Pharma innovation journal, 11(8), 1412-1415. https://doi.org/10.5772/intechopen.88012.
Chaudhary, T., Dixit, M., Gera, R., Shukla, A. K., Prakash, A., Gupta, G., & Shukla, P. (2020). Techniques for improving formulations of bioinoculants. 3 Biotech, 10(5), 1-9. https://doi.org/10.1007/s13205-020-02182-9.
Donati, A. J., Lee, H. I., Leveau, J. H., & Chang, W. S. (2013). Effects of indole-3-acetic acid on the transcriptional activities and stress tolerance of Bradyrhizobium japonicum. PloS one, 8(10), e76559. https://doi.org/10.1371/journal.pone.0076559.
Ganeshamoorthi, P., Anand, T., Prakasam, V., Bharani, M., Ragupathi, N., & Samiyappan, R. (2008). Plant growth promoting rhizobacterial (PGPR) bioconsortia mediates induction of defense-related proteins against infection of root rot pathogen in mulberry plants. Journal of Plant Interactions, 3(4), 233-244.
Glick, B. R. (2005). Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS microbiology letters, 251(1), 1-7. https://doi.org/10.1016/j.femsle.2005.07.030.
Hasani, H., & Aminpanah, H. (2015). Effect of Pseudomonas fluorescens Inoculation on Yield and Yield Components of Rice (Oryza sativa L.) under Different Levels of Phosphorus Fertilizer. Thai Journal of Agricultural Science, 48(3), 157-163.
Jaramillo, M. P., Álvarez, A. E. B., Navarrete, E. D. T., Martínez, H. F. C., Prieto-Encalada, H., & Carriel, J. M. (2016). Producción de ácido indol-3-acético por Pseudomonas veronii R4 y formación de raíces en hojas de vid “Thompson seedless” in vitro. Ciencia y Tecnología, 9(1), 31-36. https://doi.org/10.18779/cyt.v9i1.158.
Jeyanthi, V., & Kanimozhi, S. (2018). Plant growth promoting rhizobacteria (PGPR)-prospective and mechanisms: a review. J Pure Appl Microbiol, 12(2), 733-749. https://dx.doi.org/10.22207/JPAM.12.2.34.
Kalita, M., Bharadwaz, M., Dey, T., Gogoi, K., Dowarah, P., Unni, B. G., ... & Saikia, I. (2015). Developing novel bacterial based bioformulation having PGPR properties for enhanced production of agricultural crops. Indian Journal of Experimental Biology, 53(1), 56-60.
Katiyar, D., Hemantaranjan, A., & Singh, B. (2016). Plant growth promoting Rhizobacteria-an efficient tool for agriculture promotion. Adv Plants Agric Res, 4(6), 426-434.
Keswani, C., Singh, S. P., Cueto, L., García-Estrada, C., Mezaache-Aichour, S., Glare, T. R., ... & Sansinenea, E. (2020). Auxins of microbial origin and their use in agriculture. Applied Microbiology and Biotechnology, 104, 8549-8565.
King, E. O., Ward, M. K., & Raney, D. E. (1954). Two simple media for the demonstration of pyocyanin and fluorescin. The Journal of laboratory and clinical medicine, 44(2), 301-307.
Koul, B., Pudhuvai, B., Sharma, C., Kumar, A., Sharma, V., Yadav, D., & Jin, J. O. (2022). Carica papaya L.: a tropical fruit with benefits beyond the tropics. Diversity, 14(8), 683. https://doi.org/10.3390/d14080683.
Marquina, M. E., Ramírez, Y., & Castro, Y. (2018). Efecto de bacterias rizosféricas en la germinación y crecimiento del pimentón Capsicum annuum L. Var. cacique gigante. Bioagro, 30(1), 3-16.
Martínez-Viveros, O., Jorquera, M. A., Crowley, D. E., Gajardo, G. M. L. M., & Mora, M. L. (2010). Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. Journal of soil science and plant nutrition, 10(3), 293-319.
Minchev, Z., Kostenko, O., Soler, R., & Pozo, M. J. (2021). Microbial consortia for effective biocontrol of root and foliar diseases in tomato. Frontiers in Plant Science, 2428. http://dx.doi.org/10.4067/S0718-95162010000100006.
Mitra, D., Mondal, R., Khoshru, B., Shadangi, S., Mohapatra, P. K. D., & Panneerselvam, P. (2021). Rhizobacteria mediated seed bio-priming triggers the resistance and plant growth for sustainable crop production. Current Research in Microbial Sciences, 2, 100071. https://doi.org/10.1016/j.crmicr.2021.100071.
Naveed, M., Qureshi, M. A., Zahir, Z. A., Hussain, M. B., Sessitsch, A., & Mitter, B. (2015). L-Tryptophan-dependent biosynthesis of indole-3-acetic acid (IAA) improves plant growth and colonization of maize by Burkholderia phytofirmans PsJN. Annals of microbiology, 65, 1381-1389. https://doi.org/10.1007/s13213-014-0976-y.
Nikfarjam, S. G., & Aminpanah, H. (2015). Effects of phosphorus fertilization and Pseudomonas fluorescens strain on the growth and yield of faba bean (Vicia faba L.). Idesia, 33(4), 15-21. http://dx.doi.org/10.4067/S0718-34292015000400003.
Patten, C. L., & Glick, B. R. (1996). Bacterial biosynthesis of indole-3-acetic acid. Canadian journal of microbiology, 42(3), 207-220. https://doi.org/10.1139/m96-032.
Qessaoui, R., Bouharroud, R., Furze, J. N., El Aalaoui, M., Akroud, H., Amarraque, A., ... & Chebli, B. (2019). Applications of new rhizobacteria Pseudomonas isolates in agroecology via fundamental processes complementing plant growth. Scientific Reports, 9(1), 1-10. https://doi.org/10.1038/s41598-019-49216-8.
Raj, R. D. P., Preethy, H. A., & Rex, K. G. R. (2021). Development of Banana Peel Powder as Organic Carrier based Bioformulation and Determination of its Plant Growth Promoting Efficacy in Rice Cr100g. Journal of Pure and Applied Microbiology, 15(1), 1279-1290. https://doi.org/10.22207/JPAM.15.3.18.
Rodríguez, S., Vargas, I., Hijuelo, A., Loumeto, F., Silva, J. J., Pérez, J., ... & Oliva, D. (2019). Analysis of the effect of scarification process on papaya (Carica papaya Lin.) Seeds Germination. In Seed Dormancy and Germination. IntechOpen. https://doi.org/10.5772/intechopen.88012.
Romero Rodríguez, J. A., Mejía Contreras, J. A., Carballo Carballo, A., López Jiménez, A., Rangel Lucio, J. A., & Ávila Reséndiz, C. (2013). Escarificación química de semilla de papaya. Revista mexicana de ciencias agrícolas, 4(6), 947-954. https://doi.org/10.29312/remexca.v4i6.1162.
Rosa, P. A. L., Mortinho, E. S., Jalal, A., Galindo, F. S., Buzetti, S., Fernandes, G. C., ... & Teixeira Filho, M. C. M. (2020). Inoculation with growth-promoting bacteria associated with the reduction of phosphate fertilization in sugarcane. Frontiers in Environmental Science, 32(8), 1-18. https://doi.org/10.3389/fenvs.2020.00032.
Rueden, C. T., Schindelin, J., Hiner, M. C., DeZonia, B. E., Walter, A. E., Arena, E. T., & Eliceiri, K. W. (2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC bioinformatics, 18(1), 1-26. https://doi.org/10.1186/s12859-017-1934-z.
Saavedra, D. A. C., Martínez, H. F. C., Rosero, N. C., Cuenca, F. F. G., Fernández, R. G., & Moreira, Á. V. C. (2017). Rizobacterias que promueven el desarrollo e incremento en productividad de Glycine max L. Revista Ciencia y Tecnología, 10(1), 7-15. https://doi.org/10.18779/cyt.v10i1.198.
Saidi, A., & Hajibarat, Z. (2021). Phytohormones: Plant switchers in developmental and growth stages in potato. Journal of Genetic Engineering and Biotechnology, 19(1), 1-17. https://doi.org/10.1186/s43141-021-00192-5.
Sharma, N., Manhas, R. K., & Ohri, P. (2022). Streptomyces hydrogenans strain DH-16 alleviates negative impact of Meloidogyne incognita stress by modifying physio-biochemical attributes in Solanum lycopersicum plants. Scientific Reports, 12(1), 15214. https://doi.org/10.1038/s41598-022-19636-0.
Silva, J. D., Rashid, Z., Nhut, D. T., Sivakumar, D., Gera, A., Souza, M. T., & Tennant, P. (2007). Papaya (Carica papaya L.) biology and biotechnology. Tree and Forestry Science and Biotechnology, 1(1), 47-73.
Truyens, S., Weyens, N., Cuypers, A., & Vangronsveld, J. (2015). Bacterial seed endophytes: genera, vertical transmission and interaction with plants. Environmental Microbiology Reports, 7(1), 40-50. https://doi.org/10.1111/1758-2229.12181.
Tsukanova, K. A., Meyer, J. J. M., & Bibikova, T. N. (2017). Effect of plant growth-promoting Rhizobacteria on plant hormone homeostasis. South African Journal of Botany, 113, 91-102. https://doi.org/10.1016/j.sajb.2017.07.007.
Ugoji, E. O., & Laing, M. D. (2008). Rhizotron studies on Zea mays L. to evaluate biocontrol activity of Bacillus subtilis. World Journal of Microbiology and Biotechnology 24, 269-274. https://doi.org/10.1007/s11274-007-9466-8.
Vassileva, M., Malusà, E., Sas-Paszt, L., Trzcinski, P., Galvez, A., Flor-Peregrin, E., ... & Vassilev, N. (2021). Fermentation strategies to improve soil bio-inoculant production and quality. Microorganisms, 9(6), 1254. https://doi.org/10.3390/microorganisms9061254.
Vega-Celedón, P., Canchignia Martínez, H., González, M., & Seeger, M. (2016). Biosíntesis de ácido indol-3-acético y promoción del crecimiento de plantas por bacterias. Cultivos Tropicales, 37, 33-39. https://dx.doi.org/10.13140/RG.2.1.5158.3609
Vidhyasekaran, P., & Muthamilan, M. (1995). Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant disease, 79(8), 782-786. https://doi.org/10.1016/S0261-2194(96)00045-2.
Widawati, S. (2017, November). Effect of plant growth promoting rhizobacteria and molasses on seed germination and seedling growth of Sorghum bicolor L. Moench. In Proceedings The SATREPS Conference (Vol. 1, No. 1, pp. 94-99).
Wong, C. K. F., Saidi, N. B., Vadamalai, G., Teh, C. Y., & Zulperi, D. (2019). Effect of bioformulations on the biocontrol efficacy, microbial viability and storage stability of a consortium of biocontrol agents against Fusarium wilt of banana. Journal of applied microbiology, 127(2), 544-555. https://doi.org/10.1111/jam.14310.
Yang, W. (2019). Components of rhizospheric bacterial communities of barley and their potential for plant growth promotion and biocontrol of Fusarium wilt of watermelon. Brazilian Journal of Microbiology, 50(3), 749-757.
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Derechos de autor 2023 Cristhian John Macías-Holguín, Hayron Fabricio Canchignia-Martínez, Vicenta Dayana Delgado-Basurto, Fernando Patricio Paucar-Nieto, Ketty Vanessa Arellano-Ibarra, Ángel Virgilio Cedeño-Moreira
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
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