Aislamiento y caracterización molecular de Halomonas degradadores de cianuro provenientes de efluentes mineros en el Perú
DOI:
https://doi.org/10.17268/manglar.2020.049Resumen
Los efluentes cianurados de minerías es un problema ambiental, que viene siendo tratado por métodos físicos o químicos, sin embargo, estos procesos no son sostenibles para el medio ambiente. Por ello, una de las alternativas es la aplicación de tratamientos biológicos por microorganismos autóctonos. Se aislaron dos cultivos microbianos tolerantes hasta 800 ppm NaCN del género de Halomonas sp denominados JM1 y JM3. Se caracterizaron su fisiología por pruebas bioquímicas, y molecular mediante secuenciación del gen 16S ARNr. Se evaluó su capacidad biodegradativa de 150 ppm de cianuro libre (CN-) de un efluente cianurado por 7 días en medio mínimo de sales 9M de pH 10,67 en condiciones de agitación de 130 rpm a 23 °C. Se encontró que JM1 y JM3 mostraron un porcentaje de biodegradación del CN- de 24,732% ± 1,210 y 33,996% ± 1,335, respectivamente. Esto les convierte buenos candidatos para el tratamiento biológico de residuos contaminados con cianuro como alternativa a las técnicas convencionales en el Perú.
Citas
Al-Badri, B.; Al-Maawali, S.; Al-Balushi, Z.; Al-Mahmooli, I.; Al-Sadi, A.; Velazhahan, R. 2020. Cyanide degradation and antagonistic potential of endophytic Bacillus subtilis strain BEB1 from Bougainvillea spectabilis Willd. All Life 13(1): 92–98.
Alonso-González, O.; Jiménez-Velasco, C.; Nava-Alonso, F.; Alvarado-Hernández, F.; González-Anaya, J. 2017. Free cyanide analysis by silver nitrate titration with sulfide ion as interference. Minerals Engineering 105: 19–21.
Bergey, D. 2009. Bergey’s manual of systematic bacteriology: The Archaea and the Deeply Branching and Phototrophic Bacteria 1: 1771-1780.
Cardona-Giraldo, E.A. 2015. Microorganismos Potenciales degradadores de Cianuro en residuos de minería de oro. Tesis de Magister, Universidad Nacional de Colombia, Medellín. Colombia. 86 pp.
Chen, L.; Zhu, X.; Zhang, M.; Wang, Y.; Lv, T.; Zhang, S.; Yu, X. 2017. Profiling total viable bacteria in a hemodialysis water treatment system. Journal of Microbiology and Biotechnology 27(5): 995–1004.
Dash, R. R.; Gaur, A.; Balomajumder, C. 2009. Cyanide in industrial wastewaters and its removal: A review on biotreatment. Journal of Hazardous Materials 163(1): 1–11.
Gurbuz, F.; Ciftci, H.; Akcil, A. 2009. Biodegradation of cyanide containing effluents by Scenedesmus obliquus. Journal of Hazardous Materials 162(1): 74–79.
Karamba, K.I.; Ahmad, S.A.; Zulkharnain, A.; Syed, M.A.; Khalil, K.A.; Shamaan, N.A.; Dahalam, F; Shukor, M.Y. 2016. Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology. Rendiconti Lincei 27(3): 533–545.
Khamar, Z.; Makhdoumi-Kakhki, A.; Mahmudy, M.H. 2015. Remediation of cyanide from the gold mine tailing pond by a novel bacterial co-culture. International Biodeterioration and Biodegradation 99: 123–128.
Kim, K.K.; Lee, K.C.; Oh, H.M.; Lee, J.S. 2010. Halomonas stevensii sp. nov., halomonas hamiltonii sp. nov. and halomonas johnsoniae sp. nov., isolated from a renal care centre. International Journal of Systematic and Evolutionary Microbiology 60(2): 369–377.
Luque-almagro, M.; Martı, M.; Moreno-Vivia, C.; Rolda, M.D.; Jesu, L.; Castillo, F.; Blasco, R. 2005. Bacterial Degradation of Cyanide and Its Metal Complexes under Alkaline Conditions. Applied and Environmental Microbiology 71(2): 940–947.
Luque-Almagro, V.M.; Moreno-Vivián, C.; Roldán, M.D. 2016. Biodegradation of cyanide wastes from mining and jewellery industries. Current Opinion in Biotechnology 38: 9–13.
Mekuto, L.; Ntwampe, S.K.; Akcil, A. 2016. An integrated biological approach for treatment of cyanidation wastewater. Science of the Total Environment 571: 711–720.
Moradkhani, M.; Yaghmaei, S.; Nejad, Z. G. 2018. Biodegradation of cyanide under alkaline conditions by a strain of Pseudomonas putida isolated from gold mine soil and optimization of process variables through response surface methodology (RSM). Periodica Polytechnica Chemical Engineering 62(3): 265–273.
Nyamunda, B.C. 2017. Review of the Impact on Water Quality and Treatment Options of Cyanide Used in Gold Ore Processing. En: Hlanganami, T. (Comp.). Water Quality. Intechopen. South Africa. Pp. 225- 244.
Ofori-Sarpong, G.; Adam, A.; Amankwah, R. 2020. Detoxification of Cyanide Wastewater by Cyanotrophic Organisms: the case of Phanerochaete chrysosporium. Ghana Mining Journal 20(1): 34–44.
Park, J.M.; Trevor Sewell, B.; Benedik, M.J. 2017. Cyanide bioremediation: the potential of engineered nitrilases. Applied Microbiology and Biotechnology 101(8): 3029–3042.
Razanamahandry, L.C.; Andrianisa, H.A.; Karoui, H.; Kouakou, K. M.; Yacouba, H. 2016. Biodegradation of free cyanide by bacterial species isolated from cyanide-contaminated artisanal gold mining catchment area in Burkina Faso. Chemosphere 157: 71–78.
Sernaque-Aguilar, Y. A.; Cornejo-La Torre, M.; Regard, J. P.; Mialhe Matonnier, E. L. 2019. Caracterización molecular de bacterias cultivables y no cultivables procedentes de pozas de lixiviación con cianuro. Revista Peruana de Biología 26(2): 275–282.
Sharma, M.; Akhter, Y.; Chatterjee, S. 2019. A review on remediation of cyanide containing industrial wastes using biological systems with special reference to enzymatic degradation. World Journal of Microbiology and Biotechnology 35(5): 1–14.
Tang, X.; Zhai, L.; Lin, Y.; Yao, S.; Wang, L.; Ge, Y.; Liu, Y.; Zhang, X.; Zhang, T.; Zhang, L.; Liu, J.; Cheng, C. 2017. Halomonas alkalicola sp. nov., isolated from a household product plant. International Journal of Systematic and Evolutionary Microbiology 67(5): 1546–1550.
Truhlar, A.M.; Denes, T.G.; Cantilina, K.K.; Leung, S.K.; Todd Walter, M.; Hay, A.G. 2018. Absence of genetic selection in a pathogenic Escherichia coli strain exposed to the manure-amended soil environment. PLoS ONE 13(12): 1–14.
Wang, Y.X.; Xiao, W.; Dong, M.H.; Zhao, Q.; Li, Z.Y.; Lai, Y.H.; Cui, X.L. 2014. Halomonas qiaohouensis sp. nov., isolated from salt mine soil in southwest China. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 106(2): 253–260.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Manglar is an open access journal distributed under the terms and conditions of Creative Commons Attribution 4.0 International license
