Pathogenicity of Pectobacterium chrysanthemi on Solanum tuberosum and PCR of the internal transcribed spacer (ITS) 16S/23S rDNA in isolates of Musa sp. from northern Peru
DOI:
https://doi.org/10.57188/manglar.2024.032Abstract
Pectobacterium chrysanthemi is the causative agent of soft rot that commonly affects organic banana crops around the planet. Current phytosanitary control policies are based on the symptoms caused by the disease. However, since there are other species that cause similar symptoms, pathogenicity tests are used to which biochemical tests must be added to identify the bacteria, dispensing with molecular tests that, in contrast, would allow obtaining more specific results and in less time. time. This article presents an alternative methodology for the rapid and specific diagnosis of soft rot caused by Pectobacterium chrysanthemi in organic banana crops in Peru. Detection by virulence of the bacteria in host tissue of Solanum tuberosum and PCR of the internal transcribed spacer (ITS) of the 16S/23S rDNA allows timely management of the disease. The results demonstrate that in the complex between the pathogenicity test and the ITS-16S/23S PCR assay it is possible to detect Pectobacterium chrysanthemi in a period of 8 days, 3 hours and 15 minutes, demonstrating that this method is more efficient than conventional tests. biochemical tests usually used for the diagnosis of soft rot in Musa sp.
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Copyright (c) 2024 Edgar Abraham Maldonado Duque, Angélica María Vigíl-Correa, Maria Dorothy Torres de León, Roberto Mendoza Rendón, Cindy Yuriko Saavedra-Rios, Irwin David Villar Guevara, Carlos Enrique Cubas Zúñiga, Merli Clarita Peña Zarate, Karol Noemi Vilchez Estrada
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