Efecto de las condiciones de cultivo sobre el crecimiento y la producción de ácido docosahexaenoico por Aurantiochytrium limacinum cepa 85
DOI:
https://doi.org/10.17268/manglar.2015.005Resumo
El efecto de la velocidad de rotación, pH, temperatura y salinidad sobre el crecimiento y producción de ácido docosahexaenoico (DHA) en una cepa de traustoquitridio con 99% de similaridad a Aurantiochytrium limacinum fueron investigados con ensayos a tres niveles: 50, 100 y 150 rpm; 4, 7 y 9 pH; 20, 25 y 30°C; 10, 20 y 30 ppm, respectivamente. La biomasa se estimó por el peso seco de las células, y el DHA por cromatografía de gases expresados como AGT en porcentaje. Se encontró que la velocidad de rotación afecto más al crecimiento, en donde una excesiva agitación afecta el crecimiento y una deficiente agitación disminuye su tasa de crecimiento. Iguales resultados se obtuvieron en relación al pH, en donde a pH más alcalino (pH9) afecta el crecimiento más que a la producción de DHA. En cambio cuando se evaluó la temperatura, este parámetro afecto el crecimiento y la producción de DHA de manera inversa, observando que a temperaturas altas hay mayor crecimiento, pero a temperaturas bajas hay un mayor porcentaje de DHA, similares resultados se obtuvieron al evaluar diferentes niveles de la salinidad, en donde mejores crecimiento fueron a salinidades más altas, pero la producción de DHA fue ligeramente más alta a la salinidad más baja. Los resultados demuestran que el crecimiento en función de la biomasa y el porcentaje de DHA en relación los ácidos grasos totales, están estrechamente relacionados a las variaciones de los parámetros evaluados.Downloads
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