Genetic variability in Musa spp. cultivars using randomly amplified polymorphic DNA (RAPD)
DOI:
https://doi.org/10.57188/manglar.2025.019Keywords:
Cultivars, Polymorphism, Genetic diversity, PCR-RAPD, Molecular markerAbstract
The north of Peru presents favorable abiotic conditions for banana production. However, artificial selection has generated cultivars that are sometimes difficult to distinguish phenotypically, limiting the selection of specimens with resistance to pests and high productive quality. For this reason, genetic material analysis is used to identify the present genotypes. The objective was to determine the genetic variability of 9 Musa spp. cultivars using Random Amplified Polymorphic DNA (RAPD). The third leaf from each banana plant belonging to a cultivar was selected, and using sterile scissors, a portion was extracted and then dissected into small sections. DNA extraction was then performed, followed by amplification using PCR-RAPD with markers OPA-1, OPA-2, OPA-3, OPA-4, A-01, OPC-2, and OPC-15, to identify genetic variability through polymorphism. Finally, the analysis of the number of amplified polymorphic bands and their respective percentages was performed using R-Studio software, which produced a dendrogram. A total of 76 bands were amplified across the cultivars IC2, Valery, Montecristo, Cavendish Gigante, Red Dacca, Williams, Gran Enano, Gros Michel, and M. paradisiaca cv. Zapatito, of which 41 were polymorphic. The dendrogram revealed a close genetic relationship between five cultivars (Gran Enano, Williams, Montecristo, IC2, and Valery) due to the similarity in their polymorphic bands. In contrast, the cultivars Gros Michel, Red Dacca, and Zapatito showed significant genetic differentiation, not clustering together, due to the absence or greater number of polymorphic bands in each. Evidence suggests that there is significant genetic diversity among Musa spp. cultivars in northern Peru, which represents a strategic resource for genetic improvement. Therefore, the systematic integration of molecular tools into selection and certification processes is proposed, with the goal of enhancing the crop's sustainability and competitiveness in the region.
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Abekova, A. M., Yerzhebayeva, R. S., Bastaubayeva, S. O., Konusbekov, K., Bazylova, T. A., Babissekova, D. I., & Amangeldiyeva, A. A. (2022). Assessment of sugar beet genetic diversity in the Republic of Kazakhstan by using RAPD markers and agromorphological traits. SABRAO Journal of Breeding and Genetics, 54(1), 67-78. http://doi.org/10.54910/sabrao2022.54.1.7
Asniawati, M. D., & Purwantoro, A. (2019). Genetic diversity of croton (Codiaeum variegatum (L.) Rumph. ex A. Juss) and its offspring based on RAPD markers. Ilmu Pertanian (Agricultural Science), 4(2), 52–58. https://doi.org/10.22146/ipas.40899
Ávila, J. A. C., Cárdenas, C. C. C., Moreira, A. V. C., Benites, L. F. V., & Villanueva, M. S. C. (2024). Actividad antagonista de PGPR en nematodo fitoparásito Pratylenchus spp. en Musa paradisiaca (Musa acuminata× M. balbisiana) vc cavendish. Revista Alfa, 8(24), 717-728. https://orcid.org/0000-0002-7127-2818
Boonsrangsom, T., Fuenghoi, C., Premjet, D., Suvittawat, K., Ratanasut, K., & Sujipuli, K. (2023). Genetic relationships and genome verification of Thai banana cultivars using Random Amplification of Polymorphic DNA (RAPD) markers. Biodiversitas Journal of Biological Diversity, 24(7), 3758-3765 https://doi.org/10.13057/biodiv/d240713
Buitrago-Bitar, M. A., Enríquez-Valencia, A. L., Londoño-Caicedo, J. M., Muñoz-Flórez, J. E., Villegas-Estrada, B., & Santana-Fonseca, G. E. (2020). Molecular and morphological characterization of Musa spp. (Zingiberales: Musaceae) cultivars. Boletín Científico Del Centro de Museos, 24(1), 33–47. https://doi.org/10.17151/bccm.2020.24.1.2
Chen, P., Salazar, E., Fernández, H., Castro, L., Russo, A., & Vásquez, S. (2011). Detection of RAPD polymorphisms in Musa spp. with differential response to the attack of Xanthomonas campestris pv. musacearum. Trop Agronomy, 61(2), 125–132. https://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0002-192X2011000200003
Cruz Arévalo, S. D., Romero Meza, R. F., Cedeño Moreira, Á. V., Verdosoto Valencia, Á. V., Peñafiel Jaramillo, M. F., & Canchignia Martínez, H. F. (2019). Densidad estomática, contenido de clorofila y relación filogenética en 17 cultivares de Musa spp. Scientia Agropecuaria, 10(1), 47-54. http://dx.doi.org/10.17268/sci.agropecu.2019.01.05
Darmayani, S., Shofi, M., & Raharjeng, A. R. P. (2018). RAPD Analysis of the genetic diversity among accessions of micropropagation bananas from Indonesia. Journal of Physics: Conference Series, 1114(1),012137). http://dx.doi.org/10.1088/1742-6596/1114/1/012137
Flores, K. (2010). Asociatividad de los productores y cadena productiva de bananos orgánicos en el valle del río Tumbes-Perú. Revista de Ciencias Empresariales de la Universidad de San Martín de Porres, 1(1), 37–55.
Guamangate-Casillas, E. F., León-Reyes, H. A., Granja-Guerra, E., & Barriga-Medina, N. N. (2024). Evaluación de hongos entomopatógenos en el control de picudo (Metamasius hemipterus L) en el cultivo de baby banano en el cantón La Maná. Revista de Ciencias Agropecuarias, 7(14), 92-100. https://doi.org/10.56124/allpa.v7i14.0080
Hasanah, Y., Mawarni, L., Hanum, H., & Lestami, A. (2022). Genetic diversity of shallots (Allium ascalonicum L.) from several locations in North Sumatra, Indonesia based on RAPD markers. Biodiversitas Journal of Biological Diversity, 23(5), 2405-2410. https://doi.org/10.13057/biodiv/d230518
Hinge, V. R., Shaikh, I. M., Chavhan, R. L., Deshmukh, A. S., Shelake, R. M., Ghuge, S. A., & Kadam, U. S. (2022). Assessment of genetic diversity and volatile content of commercially grown banana (Musa spp.) cultivars. Scientific Reports, 12(1), 7979. https://doi.org/10.1038/s41598-022-11992-1
Kanani, P., & Shukla, Y. M. (2020). Genetic variability: physiological characteristics, pathogenicity and molecular diversity of Fusarium oxysporum f. sp. cumini infecting Cuminum cyminum L. in India. Vegetos, 33(2), 265–276. https://doi.org/10.1007/s42535-020-00104-x
Karuwal, R. L., Kasiamdari, R., & Daryono, B. S. (2024). Genetic variation of Fei banana (Musa troglodytarum L.) in Maluku Islands using RAPD markers. SABRAO J. Breed. Genet, 56(1), 101-111. http://doi.org/10.54910/sabrao2024.56.1.9
Kessel, A. (2008). Aplicación de técnicas biotecnológicas en frutales, una vía valiosa para el rescate y la conservación de estas especies. Cultivos Tropicales, 29(3), 27–37.
Lallana, V. H., & García, L. F. (2013). Effect of pretreatments on the seed viability test of Trichocentrum jonesianum (Orchidaceae). Agricultural Research, 15(2), 129–132.
Lamare, A., & Rao, S. R. (2015). Efficacy of RAPD, ISSR, and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata Colla. Physiologia Plantarum, 21(3), 349–358. https://doi.org/10.1007/s12298-015-0295-1
Loh, J. P., Kiew, R., Set, O., Gan, L. H., & Gan, Y. (2000). Amplified Fragment Length Polymorphism fingerprinting of 16 banana cultivars (Musa cvs.). Molecular Plant Pathology, 17(3), 360–366. https://doi.org/10.1006/mpev.2000.0848
Maldonado Duque, E. A., Vigíl-Correa, A. M., Zurita Chinguel, L. G., Torres de León, M. D., Mendoza Rendón, R., Saavedra-Rios, C. Y., Villar Guevara, I. D., Cubas Zúñiga, C. E., Peña Zarate, M. C., Vilchez Estrada, K. N., & Ruiz Polo, A. A. (2024). Patogenicidad de Pectobacterium chrysanthemi en Solanum tuberosum y PCR del espaciador transcrito interno (ITS) del ADNr 16S/23S en aislados de Musa sp. del norte del Perú. Manglar, 21(3), 299-304. https://doi.org/10.57188/manglar.2024.032
Martínez, G., Rey, J. C., Pichardo, R. E. P., & Manzanilla, E. E. (2020). Marchitez por Fusarium raza tropical 4: Estado actual y presencia en el continente americano. Agronomía Mesoamericana, 31(1), 259-276.
Martínez-Solórzano, G. E., & Rey-Brina, J. C. (2021). Bananas (Musa AAA): Importance, production and trade in Covid-19 times. Agronomía Mesoamericana, 32(3), 1034–1046. https://doi.org/10.15517/am.v32i3.43610
Mehendran, Y., Kartheeswaran, T., & Kodikara, N. (2022). Banana freshness identification using image processing techniques. In 7th International Conference on Business and Industrial Research (ICBIR) (pp. 347–352). https://doi.org/10.1109/ICBIR54589.2022.9786519
Nadal-Medina, R., Manzo-Sánchez, G., Orozco-Romero, J., Orozco-Santos, M., & Guzmán-González, S. (2009). Genetic diversity of bananas and plantains (Musa spp.) determined using RAPD markers. Revista Fitotecnia Mexicana, 32(1), 1–7.
Narváez, R. C., Valenzuela, B. J., Muñoz Sch, C., & Hinrichsen, R. P. (2000). Comparison of RAPD and AFLP as methods for genetic identification of Vitis based on the analysis of anonymous genomic sequences. Biotechnology Journal, 12(6), 405–412.
Organización de las Naciones Unidas para la Alimentación y la Agricultura [FAO]. (2022). Banano - Análisis del mercado 2020. Roma.
Padmalatha, K., & Prasad, M. N. V. (2007). Morphological and molecular diversity in Pterocarpus santalinus Lf-an endemic and endangered medicinal plant. Medicinal and Aromatic Plant Science and Biotechnology, 1(2), 263-273.
Palomino, C., López, C., Espejo, R., Mansilla, R., & Quispe, J. (2014). Evaluación de la diversidad genética del café (Coffea arabica L.) en Villa Rica (Perú). Ecología aplicada, 13(2), 129-134.
Paredes, Y. R. M., Cuatzo, K. M. C., Blas, M. D. J. D., & Resendiz, J. Á. M. (2024). Usos de la hoja de plátano (Musa paradisiaca L.) en la gastronomía. Revista de Gastronomía y Cocina, 3(1), 312-312. https://doi.org/10.70221/rgc.10619494
Pillay, M., Nwakanma, D. C., & Tenkouano, A. (2000). Identification of RAPD markers linked to A and B genome sequences in Musa. Genome, 43(5), 763–767. https://doi.org/10.1139/g00-038
Pillay, M., Ogundiwin, E., Nwakanma, D. C., Ude, G., & Tenkouano, A. (2001). Analysis of genetic diversity and relationships in East African banana germplasm. Theoretical and Applied Genetics, 102(6–7), 965–970. https://doi.org/10.1007/s001220000500
Pillay, M., Tenkouano, A., Ude, G., & Ortiz, R. (2004). Molecular characterization of genomes in Musa. In M. Jain & R. Swennen (Eds.), Banana improvement: Cellular, molecular biology, and induced mutations (pp. 271–287). Science Publishers.
Rachmad, F. D., Priyatmojo, A., & Widiastuti, A. (2022). Genetic diversity analysis of Exobasidium vexans causing tea blister blight in Wonosobo, Central Java, Indonesia using RAPD markers. Archives of Phytopathology and Plant Protection, 55(10), 1234-1249. https://doi.org/10.1080/03235408.2022.2086026
Reyes, B. P. A., & Cevallos, S. (2025). Hongos asociados al cultivo de banano (Musa spp.) con potencial biotecnológico para el desarrollo de inoculantes. Siembra, 12(1). https://doi.org/10.29166/siembra.v12i1.7053
Silva, A., Trujillo, I., & Vidal, M. (2009). Evaluación de la inducción de variabilidad genética en cambur 'Manzano' (Musa AAB) a través de marcadores RAPD. Agronomía Tropical, 1–9.
Singh, W. A., Singh, N. S., Devi, E. J., Handique, P. J., & Devi, H. S. (2021). Collection and characterization of banana gene pools (Musa spp.) in Manipur (NE India) using PCR–RFLP, RAPD, and ISSR markers. Brazilian Journal of Botany, 44(3), 671–684. https://doi.org/10.1007/s40415-021-00722-y
Torres, C. D., García, Á. L., Bermúdez, C., Sarría, Z., Hurtado, R. O., Delgado, E., Pérez, A., & Fernández, M. O. (2020). Morpho-agronomic and organoleptic response of five banana cultivars (Musa spp.) under field conditions. Revista Biotecnología Vegetal, 20(1), 43–50.
Vera Aviléz, M. J. (2021). Uso de Enmiendas y Activadores Biológicos para el manejo de nematodos en el cultivo de banano (Musa AAA) zona Caracol, cantón Babahoyo. Tesis de Maestría, Universidad Técnica de Babahoyo.
Wahyudi, D., Adnin, N. I., & Hapsari, L. (2020). Intraspecific varia-tion of thirteen pisang Ambon cultivars (Musa acuminata cv. AAA) from East Java and Central Java (Indonesia) based on random amplified polymorphic DNA (RAPD) marker. International Journal of Conservation Science, 11(4).
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Copyright (c) 2025 Segundo Melecio Garcia-Garcia, Jalmer Fidel Campaña-Olaya, César Augusto Mogollón-Farias, Joel Michel Riojas-Gonzales, John Henrry Rimaycuna-Ramirez, Karla Lucia Seminario-Juárez, Archi Alejandro Ruiz-Polo, José Stalyn Córdova Campos, Erick Antonio Suarez-Peña
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